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	Provedor de dados BABT (1) BJID (1) Autor Fraga,Jorge (2) Duarte,Yanisia (1) Echevarria,Eduardo (1) Fernández,Carmen (1) Fernández-Calienes,Aymé (1) Mayet,Milagro (1) Noda,Angel Alberto (1) Rodríguez,Islay (1) Rojas,Lázara (1) Sariego,Idalia (1) Mais... Palavra-chave DNA extraction (1) DsRNA virus (1) PCR (1) Ticks (1) Trichomonas vaginalis (1) Trichomonas vaginalis virus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2005 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última An Alternative and Rapid Method for the Extraction of Nucleic Acids from Ixodid Ticks by Potassium Acetate Procedure BABT Rodríguez,Islay; Fraga,Jorge; Noda,Angel Alberto; Mayet,Milagro; Duarte,Yanisia; Echevarria,Eduardo; Fernández,Carmen. Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage of their life cycles were evaluated and compared with phenol-chloroform and ammonium hydroxide methods. The most rapid and most efficient variant was validated in the DNA extraction procedure from the engorged ticks collected from bovine, canine as well as from house ticks for the screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp. The ammonium hydroxide procedure was used for non-engorged ticks. All the variants were efficient and allowed obtaining PCR-quality material according to the specific amplification of 16S rRNA gene fragment of the original tick. DNA extracted from the ticks under the study was tested by multiplex PCR... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ticks; PCR; DNA extraction. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400542 Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates BJID Fraga,Jorge; Rojas,Lázara; Sariego,Idalia; Fernández-Calienes,Aymé. Trichomonas vaginalis can be infected with double-stranded RNA (dsRNA) viruses designated T. vaginalis virus (TVV), which may have important implications for trichomonal virulence and disease pathogenesis. We tested for TVV in 40 fresh T. vaginalis isolates from Cuban patients by total extraction of nucleic acids (DNA and RNA). TVV was detected in 22 (55%) of the 40 T. vaginalis isolates. This gives an estimate of the infection rate of Cuban T. vaginalis isolates by the dsRNA virus. Future research should focus on the association between trichomonosis symptoms and the presence of TVV. Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichomonas vaginalis; Trichomonas vaginalis virus; DsRNA virus. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600012 Registros recuperados: 2 Primeira ... 1 ... Última

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